![]() Project: PCR Instrument for Point of Care Diagnostic Testing.Once the design is approved, we complete design verification work before moving the design into prototype manufacturing and volume manufacturing. Once the fluorophores and illumination source are selected, our engineers design the entire optical pathway optimized around the needs of your specific application. Our Concept Development Phase enables us to design the optical pathway successfully the first time, with minimal design iterations, enabling our customers to accelerate their product development plans. Our NPD process can meet the needs of PCR which requires meticulous end-to-end design customization. We use this basis to define the product concept and base for a design solution.Ĭustom design processes. Gray Optics’ expertise in fluorescence wavelengths and overlap sensitivities and optical instrumentation optimization can help develop a thorough understanding of our customer’s requirements. When you work with us on your PCR diagnostics project, we provide the following:Īddressing pain points. Our LED illumination expertise can offer design solutions for various instrument architectures and form factors with LED light sources replacing traditional lamp technologies. This optimization requires end-to-end optics customization from the light source to the detector. Precise fluorescence PCR testing relies on having optimized illumination and detection pathways. In addition, the design engineer must consider the effects of spectral crosstalk between fluorophores. This accuracy is vital for 4-, 5- and 6-channel fluorescence PCR instruments when there is a small overlap of fluorophores in absorption and emission wavelengths. Correctly designing the illumination and detection optics is crucial to ensuring any PCR instrument performs tests reliably and repeatedly, with high accuracy. The level of emitted fluorescence light determines the concentration of DNA through predetermined calibration models. The amplified DNA is tagged with a dye molecule (fluorophore) that, when illuminated with light of one color (wavelength), emits light of a different, red-shift color for detection. Optics plays a crucial role in the PCR process. Since the PCR cycle is highly automated, new systems can complete the entire process in about 1-hour or less. This process is typically repeated up to 40 times, resulting in more than a billion copies of the original segment of DNA. This process duplicates the original DNA, with each new molecule harboring an old and a new strand of DNA. This step separates the two intertwined strands of DNA into single strands in a process called “denaturing.” Once denatured, a Taq polymerase enzyme synthesizes two new DNA strands from the original sample. To replicate DNA through PCR, one starts by heating the original DNA sample.
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